Summary: This protocol describes a rapid and efficient feeder-, serum-, and xeno-free method for neutrophil generation from hiPSCs using ETV2 modified mRNA (mmRNA), which directs hematoendothelial programming of hiPSCs.Hematoendothelial progenitors were cultured with GM-CSF, FGF-2, and UM171 to expand myelomonocytic progenitors, followed by treatme
ABSTRACT Despite the growing number of studies on mayflies (Ephemeroptera), especially in terms of description of new species and distributional records, the knowledge generated remains scattered across specialized literature.This dispersion poses a challenge for non-specialists, such as ecologists, hindering their ability to promote studies and co
Conventional distributed-target-based polarimetric calibration algorithms estimate polarimetric distortions by assuming that the measured spatially averaged covariance matrix takes a uma sound lantern specific form.However, when the underlying surface contains targets that do not satisfy the assumptions employed by those algorithms, the averaged co
Invasive plants are non-native species that can spread rapidly, leading to detrimental economic, ecological, or environmental impact.In aquatic systems such as the Sacramento-San Joaquin River Delta in California, USA, management agencies use manned aerial vehicles (MAV) imaging spectroscopy missions to map and track annual changes in invasive aqua
Transgenic orb grinder crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach.Recessive mutations in the extracellular domain of toxin-binding cadherin proteins that confer resistance to Bt toxin Cry1Ac by disrupting toxin